› 7ohd

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF FERRIC MURINE NEUROGLOBIN CDLESS MUTANT

Released:
DOI: 10.2210/pdb7ohd/pdb
PDB entry 7ohd coloured by chain, front view.
PDB entry 7ohd coloured by chain, side view.
PDB entry 7ohd coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Probing the Role of Murine Neuroglobin CDloop-D-Helix Unit in CO Ligand Binding and Structural Dynamics.
Exertier C, Sebastiani F, Freda I, Gugole E, Cerutti G, Parisi G, Montemiglio LC, Becucci M, Viappiani C, Bruno S, Savino C, Zamparelli C, Anselmi M, Abbruzzetti S, Smulevich G, Vallone B
OpenAccess logo ACS Chem Biol 17 2099-2108 (2022)
PMID: 35797699

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-573976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuroglobin Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ER97 (Residues: 1-42, 57-151; Coverage: 91%)
Gene name: Ngb
Sequence domains: Globin

Ligands and Environments

5 bound ligands:
Ligand HEM 2 x HEM
Ligand SO4 2 x SO4
Ligand GOL 4 x GOL
Ligand IPA 2 x IPA
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P63
Unit cell:
a: 95.941Å b: 95.941Å c: 58.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Nitrite Reductase Activity of Ferrous Nitrobindins: A Comparative Study.
De Simone et al. (2023)
1 more