› 7oid

Electron Microscopy
3.7Å resolution

Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5A

Released:
DOI: 10.2210/pdb7oid/pdb
PDB entry 7oid coloured by chain, front view.
PDB entry 7oid coloured by chain, side view.
PDB entry 7oid coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A distinct assembly pathway of the human 39S late pre-mitoribosome.
Cheng J, Berninghausen O, Beckmann R
OpenAccess logo Nat Commun 12 4544 (2021)
PMID: 34315873
Related structures: EMD-12926

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 56-mer (preferred)
PDBe Complex ID:
PDB-CPX-238613 (preferred)
Entry contents:
53 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (56 distinct):
Large ribosomal subunit protein uL2m Chain: D
Molecule details ›
Chain: D
Length: 305 amino acids
Theoretical weight: 33.36 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q5T653 (Residues: 1-305; Coverage: 100%)
Gene names: CGI-22, MRPL2
Sequence domains:
Large ribosomal subunit protein uL3m Chain: E
Molecule details ›
Chain: E
Length: 348 amino acids
Theoretical weight: 38.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P09001 (Residues: 1-348; Coverage: 100%)
Gene names: MRL3, MRPL3, RPML3
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4m Chain: F
Large ribosomal subunit protein bL9m Chain: H
Large ribosomal subunit protein uL10m Chain: I
Large ribosomal subunit protein uL11m Chain: J
Large ribosomal subunit protein uL13m Chain: K
Large ribosomal subunit protein uL14m Chain: L
Large ribosomal subunit protein uL15m Chain: M
Large ribosomal subunit protein uL16m Chain: N
Large ribosomal subunit protein bL17m Chain: O
Large ribosomal subunit protein uL18m Chain: P
Large ribosomal subunit protein bL19m Chain: Q
Large ribosomal subunit protein bL20m Chain: R
Large ribosomal subunit protein bL21m Chain: S
Large ribosomal subunit protein uL22m Chain: T
Large ribosomal subunit protein uL23m Chain: U
Large ribosomal subunit protein uL24m Chain: V
Large ribosomal subunit protein bL27m Chain: W
Large ribosomal subunit protein bL28m Chain: X
Large ribosomal subunit protein uL29m Chain: Y
Large ribosomal subunit protein uL30m Chain: Z
Large ribosomal subunit protein bL32m Chain: 0
Large ribosomal subunit protein bL33m Chain: 1
Large ribosomal subunit protein bL34m Chain: 2
Large ribosomal subunit protein bL35m Chain: 3
Large ribosomal subunit protein bL36m Chain: 4
Large ribosomal subunit protein mL37 Chain: 5
Large ribosomal subunit protein mL38 Chain: 6
Large ribosomal subunit protein mL39 Chain: 7
Large ribosomal subunit protein mL40 Chain: 8
Large ribosomal subunit protein mL41 Chain: 9
Large ribosomal subunit protein mL42 Chain: a
Large ribosomal subunit protein mL43 Chain: b
Large ribosomal subunit protein mL44 Chain: c
Large ribosomal subunit protein mL45 Chain: d
Large ribosomal subunit protein mL46 Chain: e
Large ribosomal subunit protein mL48 Chain: f
Large ribosomal subunit protein mL49 Chain: g
Large ribosomal subunit protein mL50 Chain: h
Large ribosomal subunit protein mL51 Chain: i
Large ribosomal subunit protein mL52 Chain: j
Large ribosomal subunit protein mL53 Chain: k
Large ribosomal subunit protein mL54 Chain: l
Large ribosomal subunit protein mL55 Chain: m
Large ribosomal subunit protein mL63 Chain: o
Large ribosomal subunit protein mL62 Chain: p
Large ribosomal subunit protein mL64 Chain: q
Large ribosomal subunit protein mL66 Chain: r
Large ribosomal subunit protein mL65 Chain: s
Mitochondrial assembly of ribosomal large subunit protein 1 Chain: u
Mitochondrial ribosome and complex I assembly factor AltMIEF1 Chain: v
Acyl carrier protein, mitochondrial Chain: w
16S ribosomal RNA Chain: A
mitochondrial Val tRNA Chain: B
mitochondrial tRNA Chain: z

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand PNS 1 x PNS
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.7Å
Relevant EMDB volumes: EMD-12926

Quick links

Citations

6 review citations

Organization and expression of the mammalian mitochondrial genome.
Rackham et al. (2022)
5 more