› 7ojj

Electron Microscopy
3.5Å resolution

Lassa virus L protein with endonuclease and C-terminal domains in close proximity [MID-LINK]

Released:
DOI: 10.2210/pdb7ojj/pdb
PDB entry 7ojj coloured by chain, front view.
PDB entry 7ojj coloured by chain, side view.
PDB entry 7ojj coloured by chain, top view.
Source organism: Mammarenavirus lassaense
Primary publication:
Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
Kouba T, Vogel D, Thorkelsson SR, Quemin ERJ, Williams HM, Milewski M, Busch C, Günther S, Grünewald K, Rosenthal M, Cusack S
OpenAccess logo Nat Commun 12 7018 (2021)
PMID: 34857749
Related structures: EMD-12953

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase L Chain: L
Molecule details ›
Chain: L
Length: 2217 amino acids
Theoretical weight: 253.66 KDa
Source organism: Mammarenavirus lassaense
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A3S8NV63 (Residues: 1-2217; Coverage: 100%)
Gene name: L
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-12953
Expression system: Trichoplusia ni

Quick links

Citations

4 review citations

Lassa fever - the road ahead.
Garry RF. (2023)
3 more

2 mentions without citation

Revisiting Viral RNA-Dependent RNA Polymerases: Insights from Recent Structural Studies.
Ramaswamy et al. (2022)
1 more