Function and Biology Details
Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-158754 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA damage-binding protein 1
Molecule details ›
Chains: A, E, I, M
Length: 1159 amino acids
Theoretical weight: 129.39 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 1159 amino acids
Theoretical weight: 129.39 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
Q16531 (Residues: 1-1140; Coverage: 100%)
Sequence domains:
DDB1- and CUL4-associated factor 1
Cullin-4A
Molecule details ›
Chains: C, G, K, O
Length: 742 amino acids
Theoretical weight: 86.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Length: 742 amino acids
Theoretical weight: 86.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q13619 (Residues: 35-759; Coverage: 96%)
Sequence domains:
E3 ubiquitin-protein ligase RBX1
Molecule details ›
Chains: D, H, L, P
Length: 116 amino acids
Theoretical weight: 13.5 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: RING-H2 zinc finger domain
Length: 116 amino acids
Theoretical weight: 13.5 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: P62877 (Residues: 2-8, 9-108; Coverage: 99%)
Sequence domains: RING-H2 zinc finger domain
