› 7ol9

X-ray diffraction
2.9Å resolution

Crystal structure of C-terminally truncated Bacillus subtilis nucleoid occlusion protein (Noc) complexed to the Noc-binding site (NBS)

Released:
DOI: 10.2210/pdb7ol9/pdb
PDB entry 7ol9 coloured by chain, front view.
PDB entry 7ol9 coloured by chain, side view.
PDB entry 7ol9 coloured by chain, top view.
Source organisms:
Primary publication:
The CTP-binding domain is disengaged from the DNA-binding domain in a co-crystal structure of Bacillus subtilis Noc-DNA complex.
Sukhoverkov K, Jalal AB, Ault J, Sobott F, Lawson D, Le TK
J Biol Chem 103063 (2023)
PMID: 36841481

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121733 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nucleoid occlusion protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 29.57 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37524 (Residues: 1-242; Coverage: 86%)
Gene names: BSU40990, noc, yyaA
Sequence domains:
DNA (5'-D(*TP*AP*TP*TP*TP*CP*CP*CP*GP*GP*GP*AP*AP*AP*TP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 70.501Å b: 99.326Å c: 99.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 mention without citation

The CTP-binding domain is disengaged from the DNA-binding domain in a cocrystal structure of Bacillus subtilis Noc-DNA complex.
Sukhoverkov et al. (2023)