7omm

Electron Microscopy
3.4Å resolution

Cryo-EM structure of N. gonorhoeae LptDE in complex with ProMacrobodies (MBPs have not been built de novo)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-142614 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
LPS-assembly protein LptD Chain: A
Molecule details ›
Chain: A
Length: 801 amino acids
Theoretical weight: 87.66 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5F651 (Residues: 1-801; Coverage: 100%)
Gene names: NGO_1715, imp, lptD, ostA
Sequence domains: LPS transport system D
LPS-assembly lipoprotein LptE Chain: B
Molecule details ›
Chain: B
Length: 165 amino acids
Theoretical weight: 18.5 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5F9V6 (Residues: 1-159; Coverage: 100%)
Gene names: NGO_0282, lptE
Sequence domains: Lipopolysaccharide-assembly
Maltose/maltodextrin-binding periplasmic protein Chain: C
Maltose/maltodextrin-binding periplasmic protein Chain: D

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-12990
Expression system: Escherichia coli