7oni

Electron Microscopy
3.4Å resolution

Structure of Neddylated CUL5 C-terminal region-RBX2-ARIH2*

Released:

Function and Biology Details

Reactions catalysed:
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131906 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cullin-5 Chain: C
Molecule details ›
Chain: C
Length: 780 amino acids
Theoretical weight: 91.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q93034 (Residues: 1-780; Coverage: 100%)
Gene names: CUL5, VACM1
Sequence domains:
E3 ubiquitin-protein ligase ARIH2 Chain: H
Molecule details ›
Chain: H
Length: 495 amino acids
Theoretical weight: 57.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O95376 (Residues: 1-493; Coverage: 100%)
Gene names: ARI2, ARIH2, HT005, TRIAD1
Sequence domains:
RING-box protein 2 Chain: R
Ubiquitin-like protein NEDD8 Chain: N

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.4Å
Relevant EMDB volumes: EMD-12995
Expression systems:
  • Trichoplusia ni
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'