› 7os6

X-ray diffraction
1.43Å resolution

Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MP1)

Released:
DOI: 10.2210/pdb7os6/pdb
PDB entry 7os6 coloured by chain, front view.
PDB entry 7os6 coloured by chain, side view.
PDB entry 7os6 coloured by chain, top view.
Source organism: Rhizobium etli CFN 42
Primary publication:
Crystal structures of the elusive Rhizobium etli L-asparaginase reveal a peculiar active site.
Loch JI, Imiolczyk B, Sliwiak J, Wantuch A, Bejger M, Gilski M, Jaskolski M
OpenAccess logo Nat Commun 12 6717 (2021)
PMID: 34795296

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase II protein Chains: AAA, BBB, CCC, DDD
Molecule details ›
Chains: AAA, BBB, CCC, DDD
Length: 373 amino acids
Theoretical weight: 39.57 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2K0Z2 (Residues: 1-367; Coverage: 100%)
Gene names: RHE_PE00350, ansA
Sequence domains: L-asparaginase II

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand CL 4 x CL
Ligand EDO 6 x EDO
Ligand GOL 2 x GOL
Ligand EOH 1 x EOH
1 modified residue:
Ligand CSO 4 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 77.812Å b: 91.044Å c: 113.662Å
α: 90° β: 97.075° γ: 90°
R-values:
R R work R free
0.159 0.158 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Massive annotation of bacterial L-asparaginases reveals their puzzling distribution and frequent gene transfer events.
Zielezinski et al. (2022)
6 more