7ot5

Electron Microscopy
2.9Å resolution

CspA-70 cotranslational folding intermediate 1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 55-mer (preferred)
PDBe Complex ID:
PDB-CPX-498086 (preferred)
Entry contents:
50 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (55 distinct):
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: E
Molecule details ›
Chain: E
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Sequence domains: Ribosomal protein L4/L1 family
50S ribosomal protein L5 Chain: F
Molecule details ›
Chain: F
Length: 177 amino acids
Theoretical weight: 20.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L6 Chain: G
Molecule details ›
Chain: G
Length: 175 amino acids
Theoretical weight: 18.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL9 Chain: H
Molecule details ›
Chain: H
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7R1 (Residues: 1-149; Coverage: 100%)
Gene names: JW4161, b4203, rplI
Sequence domains:
Large ribosomal subunit protein uL13 Chain: I
Molecule details ›
Chain: I
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: J
Molecule details ›
Chain: J
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADY3 (Residues: 1-123; Coverage: 100%)
Gene names: JW3272, b3310, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: K
Molecule details ›
Chain: K
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02413 (Residues: 1-144; Coverage: 100%)
Gene names: JW3263, b3301, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: L
Molecule details ›
Chain: L
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)
Gene names: JW3275, b3313, rplP
Sequence domains: Ribosomal protein L16p/L10e
50S ribosomal protein L17 Chain: M
Molecule details ›
Chain: M
Length: 119 amino acids
Theoretical weight: 13.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L18 Chain: N
Molecule details ›
Chain: N
Length: 116 amino acids
Theoretical weight: 12.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L19 Chain: O
Molecule details ›
Chain: O
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L20 Chain: P
Molecule details ›
Chain: P
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL21 Chain: Q
Molecule details ›
Chain: Q
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG48 (Residues: 1-103; Coverage: 100%)
Gene names: JW3153, b3186, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein uL22 Chain: R
Molecule details ›
Chain: R
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P61175 (Residues: 1-110; Coverage: 100%)
Gene names: JW3277, b3315, eryB, rplV
Sequence domains: Ribosomal protein L22p/L17e
50S ribosomal protein L23 Chain: S
Molecule details ›
Chain: S
Length: 94 amino acids
Theoretical weight: 10.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Ribosomal protein L24 Chain: T
Molecule details ›
Chain: T
Length: 103 amino acids
Theoretical weight: 11.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL25 Chain: U
Molecule details ›
Chain: U
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P68919 (Residues: 1-94; Coverage: 100%)
Gene names: JW2173, b2185, rplY
Sequence domains: Ribosomal L25p family
50S ribosomal protein L27 Chain: V
Molecule details ›
Chain: V
Length: 81 amino acids
Theoretical weight: 8.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L28 Chain: W
Molecule details ›
Chain: W
Length: 77 amino acids
Theoretical weight: 8.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L29 Chain: X
Molecule details ›
Chain: X
Length: 62 amino acids
Theoretical weight: 7.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L30 Chain: Y
Molecule details ›
Chain: Y
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L31 Chain: Z
Molecule details ›
Chain: Z
Length: 66 amino acids
Theoretical weight: 7.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L32 Chain: a
Molecule details ›
Chain: a
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L33 Chain: b
Molecule details ›
Chain: b
Length: 52 amino acids
Theoretical weight: 6.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL34 Chain: c
Molecule details ›
Chain: c
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Sequence domains: Ribosomal protein L34
50S ribosomal protein L35 Chain: d
Molecule details ›
Chain: d
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL36A Chain: e
Molecule details ›
Chain: e
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Gene names: JW3261, b3299, rpmJ
Sequence domains: Ribosomal protein L36
30S ribosomal protein S2 Chain: f
Molecule details ›
Chain: f
Length: 225 amino acids
Theoretical weight: 25.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S3 Chain: g
Molecule details ›
Chain: g
Length: 208 amino acids
Theoretical weight: 23.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S4 Chain: h
Molecule details ›
Chain: h
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S5 Chain: i
Molecule details ›
Chain: i
Length: 156 amino acids
Theoretical weight: 16.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S6 Chain: j
Molecule details ›
Chain: j
Length: 104 amino acids
Theoretical weight: 12.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS7 Chain: k
Molecule details ›
Chain: k
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 2-152; Coverage: 84%)
Gene names: JW3303, b3341, rpsG
Sequence domains: Ribosomal protein S7p/S5e
30S ribosomal protein S8 Chain: l
Molecule details ›
Chain: l
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S9 Chain: m
Molecule details ›
Chain: m
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S10 Chain: n
Molecule details ›
Chain: n
Length: 99 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS11 Chain: o
Molecule details ›
Chain: o
Length: 117 amino acids
Theoretical weight: 12.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5I5F5V9 (Residues: 13-129; Coverage: 91%)
Gene names: DS442_20980, F2J48_22645, rpsK
Sequence domains: Ribosomal protein S11
30S ribosomal protein S12 Chain: p
Molecule details ›
Chain: p
Length: 123 amino acids
Theoretical weight: 13.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli #1/H766
Small ribosomal subunit protein uS13 Chain: q
Molecule details ›
Chain: q
Length: 116 amino acids
Theoretical weight: 12.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7LHZ5 (Residues: 2-117; Coverage: 98%)
Gene names: EC55989_3715, rpsM
Sequence domains: Ribosomal protein S13/S18
30S ribosomal protein S14 Chain: r
Molecule details ›
Chain: r
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S15 Chain: s
Molecule details ›
Chain: s
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS16 Chain: t
Molecule details ›
Chain: t
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: C3SYP2 (Residues: 1-82; Coverage: 100%)
Gene names: A2J79_004025, A5U30_004754, ABE91_013510, ACN81_10155, ACU57_00080, AWP47_02660, B6R15_004151, B6R31_004752, BANRA_01153, BANRA_03473, BB545_02595, BCB93_004648, BG944_004814, BGM66_003574, BGZ_03315, BGZ_03367, BK300_25595, BK383_10930, BKL28_002032, BMT50_17425, BMT91_21005, BRV02_004538, BTB68_003665, BTQ06_08500, BXT93_09655, BvCmsKKP061_02398, BvCmsNSP007_04560, BvCmsSIP010_01653, C0P57_003571, C1Q91_004654, C2M16_24775, C2R31_001293, C3F40_23855, C6669_15640, C719_003857, C7B02_26315, C9160_23660, C9194_20735, C9Z68_21275, CCS08_06505, CF22_004793, CG704_21555, CIG67_05880, CQ842_19340, CQ842_21890, CR538_06305, CTR35_004184, CV83915_03504, CWS33_25635, D3C88_03060, D3G36_24900, D4N09_19000, D9D43_23095, D9E49_21005, D9J61_07050, DD762_23905, DIV22_27270, DL968_07140, DNQ45_01400, DNX30_24590, DS732_19205, DTL43_21645, DU321_24440, E2865_03661, E4K51_10645, E5H86_23225, E6D34_21955, EAI46_08750, EAI46_19150, ECs3472, EIA08_06550, EIMP300_31950, EIZ93_15670, EN85_004117, EPS76_07525, EPS97_18825, EWK56_06055, ExPECSC038_01348, F7F11_01840, F7N46_19970, F9413_21435, F9461_09670, F9B07_09610, FGAF848_35260, FIJ20_21775, FJQ40_23010, FKO60_26240, FOI11_00085, FOI11_023095, FPI65_16330, FPS11_21845, FVB16_19505, FWK02_06545, FZU14_20420, G3V95_09900, G4A38_21035, G4A47_01280, G5603_07605, GAI89_21540, GAJ12_00085, GGB84_004394, GKF66_23925, GNW61_16330, GNZ05_25610, GOP25_24505, GP711_05970, GP944_18595, GP965_09730, GP975_09855, GP979_11870, GQE86_17960, GQM04_19120, GQM13_06275, GQM21_13480, GQN24_22215, GQN34_14095, GRW05_07330, GRW24_07355, GTP92_23395, GUC01_18090, HEP30_021265, HEP34_004233, HHH44_004385, HI055_003780, HIE29_001459, HJQ60_000018, HKA49_004932, HLX92_22510, HLZ39_14795, HLZ50_20265, HMV95_03235, HV109_05690, HV209_12690, HVV39_05045, HVW04_21985, HVW43_23290, HVY77_06760, I6H00_24675, I6H02_08660, IFC14_000812, IH772_08265, J0541_004264, J5U05_003371, J8F57_004160, JNA68_17650, JNP96_20830, NCTC10082_03675, NCTC10089_01224, NCTC10418_01999, NCTC10429_01986, NCTC10764_00466, NCTC10767_02511, NCTC10865_01552, NCTC10974_01459, NCTC11112_07458, NCTC11126_05241, NCTC11181_03429, NCTC11341_01001, NCTC13148_02463, NCTC7922_00783, NCTC7927_01359, NCTC8009_02720, NCTC8179_06855, NCTC8333_01403, NCTC8500_01313, NCTC8959_02554, NCTC8960_03978, NCTC8985_06048, NCTC9044_00378, NCTC9045_01451, NCTC9073_04663, NCTC9075_01815, NCTC9077_01607, NCTC9081_05689, NCTC9702_01486, NCTC9706_03497, NCTC9962_00822, NOI85_23935, NY836_09555, OFN31_20550, OGM49_18375, P6223_004685, PWL68_001675, Q2V20_22835, Q2V64_24865, QDW62_06175, QO046_09520, R8G00_07990, R8O40_004589, RZR61_19260, SAMEA3472044_03967, SAMEA3472056_03346, SAMEA3752557_04552, V9Z47_06540, WR15_13330, rpsP
Sequence domains: Ribosomal protein S16
30S ribosomal protein S17 Chain: u
Molecule details ›
Chain: u
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S18 Chain: v
Molecule details ›
Chain: v
Length: 66 amino acids
Theoretical weight: 7.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Ribosomal protein S19 Chain: w
Molecule details ›
Chain: w
Length: 83 amino acids
Theoretical weight: 9.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S20 Chain: x
Molecule details ›
Chain: x
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S21 Chain: y
Molecule details ›
Chain: y
Length: 70 amino acids
Theoretical weight: 8.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Cold shock protein CspB Chain: B
Molecule details ›
Chain: B
Length: 66 amino acids
Theoretical weight: 7.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
23S ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 2903 nucleotides
Theoretical weight: 941.53 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
16S ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 1534 nucleotides
Theoretical weight: 497.4 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
5S ribosomal RNA Chain: 3
Molecule details ›
Chain: 3
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Sequence domains: 5S ribosomal RNA
Messenger RNA Chain: 4
Molecule details ›
Chain: 4
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
tRNA-Leu Chain: z
Molecule details ›
Chain: z
Length: 85 nucleotides
Theoretical weight: 27.61 KDa
Sequence domains: tRNA

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-13055
Expression systems:
  • Escherichia coli
  • Escherichia coli #1/H766