› 7ot9

Electron Microscopy
2.8Å resolution

Structure of the AI-2 exporter family protein YdiK from E. coli

Released:
DOI: 10.2210/pdb7ot9/pdb
PDB entry 7ot9 coloured by chain, front view.
PDB entry 7ot9 coloured by chain, side view.
PDB entry 7ot9 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism.
Khera R, Mehdipour AR, Bolla JR, Kahnt J, Welsch S, Ermler U, Muenke C, Robinson CV, Hummer G, Xie H, Michel H
OpenAccess logo EMBO J e109990 (2022)
PMID: 35698912
Related structures: EMD-13057

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative transport protein YdiK Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 370 amino acids
Theoretical weight: 39.87 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFS7 (Residues: 1-370; Coverage: 100%)
Gene names: JW1678, b1688, ydiK
Sequence domains: AI-2E family transporter

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-13057
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Mass spectrometry of intact membrane proteins: shifting towards a more native-like context.
Oluwole et al. (2023)
1 more

1 mention without citation

Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism.
Khera et al. (2022)