› 7otc

Electron Microscopy
2.9Å resolution

Cryo-EM structure of an Escherichia coli 70S ribosome in complex with elongation factor G and the antibiotic Argyrin B

Released:
DOI: 10.2210/pdb7otc/pdb
PDB entry 7otc coloured by chain, front view.
PDB entry 7otc coloured by chain, side view.
PDB entry 7otc coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
The cyclic octapeptide antibiotic argyrin B inhibits translation by trapping EF-G on the ribosome during translocation.
Wieland M, Holm M, Rundlet EJ, Morici M, Koller TO, Maviza TP, Pogorevc D, Osterman IA, Müller R, Blanchard SC, Wilson DN
OpenAccess logo Proc Natl Acad Sci U S A 119 e2114214119 (2022)
PMID: 35500116
Related structures: EMD-13058

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 52-mer (preferred)
PDBe Complex ID:
PDB-CPX-249453 (preferred)
Entry contents:
49 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (52 distinct):
Small ribosomal subunit protein uS2 Chain: b
Molecule details ›
Chain: b
Length: 226 amino acids
Theoretical weight: 25.2 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NFK2 (Residues: 4-229; Coverage: 94%)
Gene names: ECBD_3450, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: c
Molecule details ›
Chain: c
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4K1 (Residues: 2-207; Coverage: 88%)
Gene names: ECBD_0437, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: d
Molecule details ›
Chain: d
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N548 (Residues: 2-206; Coverage: 100%)
Gene names: ECBD_0455, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: e
Molecule details ›
Chain: e
Length: 157 amino acids
Theoretical weight: 16.53 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6Z9 (Residues: 10-166; Coverage: 94%)
Gene names: ECBD_0448, rpsE
Sequence domains:
Small ribosomal subunit protein bS6 Chain: f
Molecule details ›
Chain: f
Length: 106 amino acids
Theoretical weight: 12.33 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NGG7 (Residues: 1-106; Coverage: 81%)
Gene names: ECBD_3834, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: g
Molecule details ›
Chain: g
Length: 156 amino acids
Theoretical weight: 17.62 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6W8 (Residues: 2-156; Coverage: 99%)
Gene names: ECBD_0408, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: h
Molecule details ›
Chain: h
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N537 (Residues: 2-130; Coverage: 99%)
Gene names: ECBD_0445, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: i
Molecule details ›
Chain: i
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N2Z9 (Residues: 4-130; Coverage: 98%)
Gene names: ECBD_0517, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: j
Molecule details ›
Chain: j
Length: 99 amino acids
Theoretical weight: 11.33 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6Y5 (Residues: 4-102; Coverage: 96%)
Gene names: ECBD_0430, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: k
Molecule details ›
Chain: k
Length: 117 amino acids
Theoretical weight: 12.49 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7L9 (Residues: 13-129; Coverage: 91%)
Gene names: ECBD_0454, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: l
Molecule details ›
Chain: l
Length: 123 amino acids
Theoretical weight: 13.68 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4H3 (Residues: 2-124; Coverage: 99%)
Gene names: ECBD_0407, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: m
Molecule details ›
Chain: m
Length: 115 amino acids
Theoretical weight: 12.74 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5B4 (Residues: 2-116; Coverage: 98%)
Gene names: ECBD_0453, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14 Chain: n
Molecule details ›
Chain: n
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7K8 (Residues: 1-101; Coverage: 100%)
Gene names: ECBD_0444, rpsN
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: o
Molecule details ›
Chain: o
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N811 (Residues: 2-89; Coverage: 99%)
Gene names: ECBD_0575, rpsO
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: p
Molecule details ›
Chain: p
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7D8 (Residues: 1-82; Coverage: 100%)
Gene names: ECBD_1077, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: q
Molecule details ›
Chain: q
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6Z6 (Residues: 4-83; Coverage: 95%)
Gene names: ECBD_0440, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18 Chain: r
Molecule details ›
Chain: r
Length: 67 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NGH1 (Residues: 8-74; Coverage: 89%)
Gene names: ECBD_3832, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: s
Molecule details ›
Chain: s
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N528 (Residues: 1-92; Coverage: 100%)
Gene names: ECBD_0435, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS20 Chain: t
Molecule details ›
Chain: t
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NFU3 (Residues: 2-87; Coverage: 99%)
Gene names: ECBD_3593, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein bS21 Chain: u
Molecule details ›
Chain: u
Length: 70 amino acids
Theoretical weight: 8.39 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N8B4 (Residues: 2-71; Coverage: 99%)
Gene names: ECBD_0676, rpsU
Sequence domains: Ribosomal protein S21
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7J1 (Residues: 2-272; Coverage: 99%)
Gene names: ECBD_0434, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N3G7 (Residues: 1-209; Coverage: 100%)
Gene names: ECBD_0431, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: E
Molecule details ›
Chain: E
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5K8 (Residues: 1-201; Coverage: 100%)
Gene names: ECBD_0432, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL5 Chain: F
Molecule details ›
Chain: F
Length: 177 amino acids
Theoretical weight: 20.07 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5A3 (Residues: 2-178; Coverage: 99%)
Gene names: ECBD_0443, rplE
Sequence domains:
Large ribosomal subunit protein uL6 Chain: G
Molecule details ›
Chain: G
Length: 176 amino acids
Theoretical weight: 18.8 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N2T1 (Residues: 2-177; Coverage: 99%)
Gene names: ECBD_0446, rplF
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein uL13 Chain: J
Molecule details ›
Chain: J
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N598 (Residues: 1-142; Coverage: 100%)
Gene names: ECBD_0516, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: K
Molecule details ›
Chain: K
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N3H4 (Residues: 1-123; Coverage: 100%)
Gene names: ECBD_0441, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: L
Molecule details ›
Chain: L
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N711 (Residues: 1-144; Coverage: 100%)
Gene names: ECBD_0450, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: M
Molecule details ›
Chain: M
Length: 136 amino acids
Theoretical weight: 15.33 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6Z2 (Residues: 1-136; Coverage: 100%)
Gene names: ECBD_0438, rplP
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein bL17 Chain: N
Molecule details ›
Chain: N
Length: 120 amino acids
Theoretical weight: 13.72 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4M0 (Residues: 1-120; Coverage: 95%)
Gene names: ECBD_0457, rplQ
Sequence domains: Ribosomal protein L17
Large ribosomal subunit protein uL18 Chain: O
Molecule details ›
Chain: O
Length: 116 amino acids
Theoretical weight: 12.66 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4L0 (Residues: 2-117; Coverage: 99%)
Gene names: ECBD_0447, rplR
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Large ribosomal subunit protein bL19 Chain: P
Molecule details ›
Chain: P
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6T7 (Residues: 2-115; Coverage: 99%)
Gene names: ECBD_1080, rplS
Sequence domains: Ribosomal protein L19
Large ribosomal subunit protein bL20 Chain: Q
Molecule details ›
Chain: Q
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NBS1 (Residues: 2-118; Coverage: 99%)
Gene names: ECBD_1929, rplT
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein bL21 Chain: R
Molecule details ›
Chain: R
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5D7 (Residues: 1-103; Coverage: 100%)
Gene names: ECBD_0556, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein uL22 Chain: S
Molecule details ›
Chain: S
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N2S3 (Residues: 1-110; Coverage: 100%)
Gene names: ECBD_0436, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL23 Chain: T
Molecule details ›
Chain: T
Length: 93 amino acids
Theoretical weight: 10.55 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6EGE8 (Residues: 1-93; Coverage: 93%)
Gene names: ECBD_0433, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: U
Molecule details ›
Chain: U
Length: 102 amino acids
Theoretical weight: 11.08 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5L7 (Residues: 2-103; Coverage: 98%)
Gene names: ECBD_0442, rplX
Sequence domains:
Large ribosomal subunit protein bL25 Chain: V
Molecule details ›
Chain: V
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N846 (Residues: 1-94; Coverage: 100%)
Gene names: ECBD_1472, rplY
Sequence domains: Ribosomal L25p family
Large ribosomal subunit protein bL27 Chain: W
Molecule details ›
Chain: W
Length: 84 amino acids
Theoretical weight: 9.02 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N340 (Residues: 2-85; Coverage: 99%)
Gene names: ECBD_0557, rpmA
Sequence domains: Ribosomal L27 protein
Large ribosomal subunit protein bL28 Chain: X
Molecule details ›
Chain: X
Length: 77 amino acids
Theoretical weight: 8.9 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N3L9 (Residues: 2-78; Coverage: 99%)
Gene names: ECBD_0089, rpmB
Sequence domains: Ribosomal L28 family
Large ribosomal subunit protein uL29 Chain: Y
Molecule details ›
Chain: Y
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7F5 (Residues: 1-63; Coverage: 100%)
Gene names: ECBD_0439, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein uL30 Chain: Z
Molecule details ›
Chain: Z
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7G4 (Residues: 2-59; Coverage: 98%)
Gene names: ECBD_0449, rpmD
Sequence domains: Ribosomal protein L30p/L7e
Large ribosomal subunit protein bL31 Chain: 5
Molecule details ›
Chain: 5
Length: 67 amino acids
Theoretical weight: 7.61 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140SS71 (Residues: 1-67; Coverage: 96%)
Gene names: ECBD_4088, rpmE
Sequence domains: Ribosomal protein L31
Large ribosomal subunit protein bL32 Chain: 0
Molecule details ›
Chain: 0
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NAZ2 (Residues: 2-57; Coverage: 98%)
Gene names: ECBD_2512, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33 Chain: 1
Molecule details ›
Chain: 1
Length: 50 amino acids
Theoretical weight: 5.81 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5Z7 (Residues: 4-53; Coverage: 91%)
Gene names: ECBD_0090, rpmG
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: 2
Molecule details ›
Chain: 2
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NHV0 (Residues: 1-46; Coverage: 100%)
Gene names: ECBD_4330, rpmH
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: 3
Molecule details ›
Chain: 3
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N9E3 (Residues: 2-65; Coverage: 99%)
Gene names: ECBD_1928, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein bL36 Chain: 4
Molecule details ›
Chain: 4
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5M6 (Residues: 1-38; Coverage: 100%)
Gene names: ECBD_0452, rpmJ
Sequence domains: Ribosomal protein L36
Elongation factor G Chain: w
Molecule details ›
Chain: w
Length: 704 amino acids
Theoretical weight: 77.57 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7C7 (Residues: 2-704; Coverage: 100%)
Gene names: ECBD_0409, fusA
Sequence domains:
16S ribosomal RNA Chain: a
Molecule details ›
Chain: a
Length: 1533 nucleotides
Theoretical weight: 497.06 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 2903 nucleotides
Theoretical weight: 941.51 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

7 bound ligands:
Ligand MG 292 x MG
Ligand ATP 1 x ATP
Ligand PUT 8 x PUT
Ligand SPD 2 x SPD
Ligand ZN 2 x ZN
Ligand GDP 1 x GDP
Ligand 1I7 1 x 1I7
18 modified residues:
Ligand PSU 10 x PSU
Ligand G7M 2 x G7M
Ligand 2MG 4 x 2MG
Ligand 5MC 3 x 5MC
Ligand 4OC 1 x 4OC
Ligand UR3 1 x UR3
Ligand MA6 2 x MA6
Ligand D2T 1 x D2T
Ligand 1MG 1 x 1MG
Ligand 5MU 2 x 5MU
Ligand 6MZ 2 x 6MZ
Ligand 3TD 1 x 3TD
Ligand OMG 1 x OMG
Ligand H2U 1 x H2U
Ligand OMC 1 x OMC
Ligand 2MA 1 x 2MA
Ligand OMU 1 x OMU
Ligand 4D4 1 x 4D4

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-13058

Quick links

Citations

1 review citation

Active substances of myxobacteria against plant diseases and their action mechanisms.
Zhang et al. (2023)
3 other articles