› 7otr

X-ray diffraction
2.25Å resolution

Crystal structure of a psychrophilic CCA-adding enzyme determined by SAD phasing

Released:
DOI: 10.2210/pdb7otr/pdb
PDB entry 7otr coloured by chain, front view.
PDB entry 7otr coloured by chain, side view.
PDB entry 7otr coloured by chain, top view.
Source organism: Planococcus halocryophilus
Primary publication:
CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus.
de Wijn R, Rollet K, Ernst FGM, Wellner K, Betat H, Mörl M, Sauter C
OpenAccess logo Comput Struct Biotechnol J 19 5845-5855 (2021)
PMID: 34765099

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCA-adding protein Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 48.64 KDa
Source organism: Planococcus halocryophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1C7DQ98 (Residues: 1-377; Coverage: 100%)
Gene name: BBI08_05760
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 2 x GOL
Ligand ACT 3 x ACT
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P43212
Unit cell:
a: 69.79Å b: 69.79Å c: 291.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Genomic analyses reveal a low-temperature adapted clade in Halorubrum, a widespread haloarchaeon across global hypersaline environments.
Chen et al. (2023)

1 mention without citation

CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus.
de Wijn et al. (2021)