› 7ou4

Electron Microscopy
3.3Å resolution

The structure of MutS bound to one molecule of ATP and one molecule of ADP

Released:
DOI: 10.2210/pdb7ou4/pdb
PDB entry 7ou4 coloured by chain, front view.
PDB entry 7ou4 coloured by chain, side view.
PDB entry 7ou4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cryogenic electron microscopy structures reveal how ATP and DNA binding in MutS coordinates sequential steps of DNA mismatch repair.
Borsellini A, Kunetsky V, Friedhoff P, Lamers MH
Nat Struct Mol Biol 29 59-66 (2022)
PMID: 35013597
Related structures: EMD-13074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 806 amino acids
Theoretical weight: 90.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ATP 1 x ATP
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-13074
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)
1 more