› 7ow0

X-ray diffraction
1.55Å resolution

1.55 A crystal structure of DNA/2'-O-methyl-RNA heteroduplex with overhangs solved by Zn-SAD.

Released:
DOI: 10.2210/pdb7ow0/pdb
PDB entry 7ow0 coloured by chain, front view.
PDB entry 7ow0 coloured by chain, side view.
PDB entry 7ow0 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
First High-Resolution Crystal Structures of DNA:2'-O-Methyl-RNA Heteroduplexes
Dolot R, Maciaszek A, Mikolajczyk B, Nawrot B
Crystals (2022)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121756 (preferred)
Entry contents:
2 distinct DNA molecules
Macromolecules (2 distinct):
DNA (5'-D(*TP*CP*TP*CP*CP*TP*AP*GP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 9 nucleotides
Theoretical weight: 2.71 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*(OMC)P*(OMU)P*(A2M)P*(OMG)P*(OMG)P*(A2M)P*(OMG)P*(A2M)P*(OMC))-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 3.02 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
4 modified residues:
Ligand OMC 2 x OMC
Ligand OMU 1 x OMU
Ligand A2M 3 x A2M
Ligand OMG 3 x OMG

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P43212
Unit cell:
a: 31.85Å b: 31.85Å c: 91.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.251
Expression system: Not provided

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