› 7ox8

X-ray diffraction
2.75Å resolution

Target-bound SpCas9 complex with TRAC full RNA guide

Released:
DOI: 10.2210/pdb7ox8/pdb
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PDB entry 7ox8 coloured by chain, front view.
PDB entry 7ox8 coloured by chain, side view.
PDB entry 7ox8 coloured by chain, top view.
Source organisms:
Primary publication:
Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells.
Donohoue PD, Pacesa M, Lau E, Vidal B, Irby MJ, Nyer DB, Rotstein T, Banh L, Toh MS, Gibson J, Kohrs B, Baek K, Owen ALG, Slorach EM, van Overbeek M, Fuller CK, May AP, Jinek M, Cameron P
Mol Cell 81 3637-3649.e5 (2021)
PMID: 34478654

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121758 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1372 amino acids
Theoretical weight: 158.88 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
TRAC target DNA strand Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.57 KDa
Source organism: synthetic construct
Expression system: Not provided
TRAC non-target DNA strand Chain: D
sgRNA Chain: A

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand K 12 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 178.177Å b: 67.549Å c: 187.854Å
α: 90° β: 111.23° γ: 90°
R-values:
R R work R free
0.211 0.209 0.248
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

6 review citations

Structural biology of CRISPR-Cas immunity and genome editing enzymes.
Wang et al. (2022)
5 more

1 mention without citation

Structural basis for Cas9 off-target activity.
Pacesa et al. (2022)

PDB-REDO

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