› 7oxa

X-ray diffraction
2.15Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113268 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct DNA/RNA hybrid molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1372 amino acids
Theoretical weight: 158.88 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
AAVS1 target DNA strand Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.45 KDa
Source organism: synthetic construct
Expression system: Not provided
AAVS1 non-target DNA strand Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.75 KDa
Source organism: synthetic construct
Expression system: Not provided
chimeric RNA-DNA guide Chain: A
Molecule details ›
Chain: A
Length: 81 nucleotides
Theoretical weight: 26.12 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand K 14 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 177.926Å b: 67.574Å c: 189.232Å
α: 90° β: 112.523° γ: 90°
R-values:
R R work R free
0.199 0.198 0.226
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Structural biology of CRISPR-Cas immunity and genome editing enzymes.
Wang et al. (2022)
5 more