› 7oyw

X-ray diffraction
1.28Å resolution

E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88L S247D in complex with spermidine

Released:
DOI: 10.2210/pdb7oyw/pdb
PDB entry 7oyw coloured by chain, front view.
PDB entry 7oyw coloured by chain, side view.
PDB entry 7oyw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Fine-tuning spermidine binding modes in the putrescine binding protein PotF.
Kröger P, Shanmugaratnam S, Scheib U, Höcker B
OpenAccess logo J Biol Chem 297 101419 (2021)
PMID: 34801550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putrescine-binding periplasmic protein PotF Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 39.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31133 (Residues: 27-370; Coverage: 100%)
Gene names: JW0838, b0854, potF
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

6 bound ligands:
Ligand SPD 2 x SPD
Ligand ONT 1 x ONT
Ligand ONW 1 x ONW
Ligand 3IE 4 x 3IE
Ligand CL 4 x CL
Ligand JFN 1 x JFN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 116.881Å b: 71.285Å c: 92.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.132 0.161
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Retracing the evolution of a modern periplasmic binding protein.
Michel et al. (2023)
1 more

1 mention without citation

Fine-tuning spermidine binding modes in the putrescine binding protein PotF.
Kröger et al. (2021)