› 7ozt

X-ray diffraction
1.74Å resolution

Nanobodies restore stability to cancer-associated mutants of tumor suppressor protein p16INK4a

Released:
DOI: 10.2210/pdb7ozt/pdb
PDB entry 7ozt coloured by chain, front view.
PDB entry 7ozt coloured by chain, side view.
PDB entry 7ozt coloured by chain, top view.
Source organisms:
Entry authors: Pastok MW, Burbidge O, Itzhaki L, Endicott JA, Noble MEM

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211208 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Camelid nanobody NB09 Chain: AAA
Molecule details ›
Chain: AAA
Length: 132 amino acids
Theoretical weight: 14.21 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Cyclin-dependent kinase inhibitor 2A Chain: BBB
Molecule details ›
Chain: BBB
Length: 175 amino acids
Theoretical weight: 18.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42771 (Residues: 1-156; Coverage: 100%)
Gene names: CDKN2, CDKN2A, MTS1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 42.119Å b: 182.931Å c: 65.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links