› 7p02

Electron Microscopy
2.87Å resolution

Human Neurokinin 1 receptor (NK1R) substance P Gs complex

Released:
DOI: 10.2210/pdb7p02/pdb
PDB entry 7p02 coloured by chain, front view.
PDB entry 7p02 coloured by chain, side view.
PDB entry 7p02 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of neurokinin 1 receptor in complex with Gq and Gs proteins reveal substance P binding mode and unique activation features.
Thom C, Ehrenmann J, Vacca S, Waltenspühl Y, Schöppe J, Medalia O, Plückthun A
OpenAccess logo Sci Adv 7 eabk2872 (2021)
PMID: 34878828
Related structures: EMD-13141

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210787 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Antibody fragment scFv16 Chain: H
Molecule details ›
Chain: H
Length: 298 amino acids
Theoretical weight: 32.41 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 181-181, 229-242; Coverage: 1%)
  • Canonical: P63092 (Residues: 205-251, 266-394; Coverage: 45%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Substance-P receptor Chain: R
Molecule details ›
Chain: R
Length: 382 amino acids
Theoretical weight: 44.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P25103 (Residues: 1-335; Coverage: 82%)
Gene names: NK1R, TAC1R, TACR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Substance P Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P20366 (Residues: 58-68; Coverage: 10%)
Gene names: NKA, NKNA, TAC1, TAC2
Sequence domains: Tachykinin family

Ligands and Environments

1 bound ligand:
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.87Å
Relevant EMDB volumes: EMD-13141
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
7 more

2 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
1 more