› 7p0y

X-ray diffraction
2.25Å resolution

Crystal Structure of mtbMGL K74A (Substrate Analog Complex)

Released:
DOI: 10.2210/pdb7p0y/pdb
PDB entry 7p0y coloured by chain, front view.
PDB entry 7p0y coloured by chain, side view.
PDB entry 7p0y coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural Changes in the Cap of Rv0183/mtbMGL Modulate the Shape of the Binding Pocket.
Grininger C, Leypold M, Aschauer P, Pavkov-Keller T, Riegler-Berket L, Breinbauer R, Oberer M
OpenAccess logo Biomolecules 11 (2021)
PMID: 34572512

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 30.64 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O07427 (Residues: 1-279; Coverage: 100%)
Gene name: Rv0183
Sequence domains: Serine aminopeptidase, S33

Ligands and Environments

1 bound ligand:
Ligand 4E0 2 x 4E0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Unit cell:
a: 74.69Å b: 82.603Å c: 93.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.253 0.295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The cause-effect relation of tuberculosis on incidence of diabetes mellitus.
Bisht et al. (2023)
1 other article

1 mention without citation

Structural Changes in the Cap of Rv0183/mtbMGL Modulate the Shape of the Binding Pocket.
Grininger et al. (2021)