› 7p1s

X-ray diffraction
1.92Å resolution

Structure of KDNase from Trichophyton Rubrum in complex with 2,3-didehydro-2,3-dideoxy-D-glycero-D-galacto-nonulosonic acid.

Released:
DOI: 10.2210/pdb7p1s/pdb
PDB entry 7p1s coloured by chain, front view.
PDB entry 7p1s coloured by chain, side view.
PDB entry 7p1s coloured by chain, top view.
Source organism: Trichophyton rubrum
Primary publication:
Kinetic and Structural Characterization of Sialidases (Kdnases) from Ascomycete Fungal Pathogens.
Nejatie A, Steves E, Gauthier N, Baker J, Nesbitt J, McMahon SA, Oehler V, Thornton NJ, Noyovitz B, Khazaei K, Byers BW, Zandberg WF, Gloster TM, Moore MM, Bennet AJ
ACS Chem Biol 16 2632-2640 (2021)
PMID: 34724608

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 386 amino acids
Theoretical weight: 42.21 KDa
Source organism: Trichophyton rubrum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A178EUH2 (Residues: 23-408; Coverage: 100%)
Gene name: A7C99_5399
Sequence domains: BNR repeat-like domain

Ligands and Environments

2 bound ligands:
Ligand KFN 4 x KFN
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 48.646Å b: 179.008Å c: 97.292Å
α: 90° β: 104.04° γ: 90°
R-values:
R R work R free
0.261 0.26 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Structure of the immunoregulatory sialidase NEU1.
Gorelik et al. (2023)
1 more