› 7p3x

Electron Microscopy
9.1Å resolution

Homology model of the full-length AP-3 complex in a compact open conformation

Released:
DOI: 10.2210/pdb7p3x/pdb
PDB entry 7p3x coloured by chain, front view.
PDB entry 7p3x coloured by chain, side view.
PDB entry 7p3x coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Flexible open conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi.
Schoppe J, Schubert E, Apelbaum A, Yavavli E, Birkholz O, Stephanowitz H, Han Y, Perz A, Hofnagel O, Liu F, Piehler J, Raunser S, Ungermann C
OpenAccess logo J Biol Chem 297 101334 (2021)
PMID: 34688652
Related structures: EMD-13187

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153558 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
AP-3 complex subunit delta Chain: A
Molecule details ›
Chain: A
Length: 964 amino acids
Theoretical weight: 110.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q08951 (Residues: 1-932; Coverage: 100%)
Gene names: APL5, YKS4, YPL195W
Sequence domains: Adaptin N terminal region
AP-3 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 809 amino acids
Theoretical weight: 91.71 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P46682 (Residues: 1-809; Coverage: 100%)
Gene names: APL6, G9331, YGR261C, YKS5
Sequence domains: Adaptin N terminal region
AP-3 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 194 amino acids
Theoretical weight: 21.95 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P47064 (Residues: 1-194; Coverage: 100%)
Gene names: APS3, J1274, YJL024C, YKS7
Sequence domains: Clathrin adaptor complex small chain
AP-3 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 483 amino acids
Theoretical weight: 54.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38153 (Residues: 1-483; Coverage: 100%)
Gene names: APM3, YBR2035, YBR288C, YKS6
Sequence domains: Adaptor complexes medium subunit family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.1Å
Relevant EMDB volumes: EMD-13187
Expression system: Saccharomyces cerevisiae

Quick links

Citations

4 citation in other articles

Yeast TLDc domain proteins regulate assembly state and subcellular localization of the V-ATPase.
Klössel et al. (2024)
3 more

1 mention without citation

AP-3 shows off its flexibility for the cryo-EM camera.
Graham TR. (2022)