› 7p5b

X-ray diffraction
1.13Å resolution

Variant Surface Glycoprotein 3 (VSG3, MiTat1.3, VSG224) mutant (serine 319 to alanine), single O-linked glycosylated at Ser317

Released:
DOI: 10.2210/pdb7p5b/pdb
PDB entry 7p5b coloured by chain, front view.
PDB entry 7p5b coloured by chain, side view.
PDB entry 7p5b coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Immunodominant surface epitopes power immune evasion in the African trypanosome.
Gkeka A, Aresta-Branco F, Triller G, Vlachou EP, van Straaten M, Lilic M, Olinares PDB, Perez K, Chait BT, Blatnik R, Ruppert T, Verdi JP, Stebbins CE, Papavasiliou FN
Cell Rep 42 112262 (2023)
PMID: 36943866

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Variant surface glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 53.69 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Trypanosoma brucei brucei
UniProt:
  • Canonical: B3GVK1 (Residues: 1-508; Coverage: 100%)
Gene names: Tb427.BES153.14, Tb427.BES65.13
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand GLC 1 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I213
Unit cell:
a: 129.007Å b: 129.007Å c: 129.007Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.198
Expression system: Trypanosoma brucei brucei

Quick links

Citations

2 citation in other articles

A trypanosome-derived immunotherapeutics platform elicits potent high-affinity antibodies, negating the effects of the synthetic opioid fentanyl.
Triller et al. (2023)
1 more