› 7p6j

X-ray diffraction
1.75Å resolution

Crystal structure of glycosyl-enzyme intermediate of RBcel1 Y201F

Released:
DOI: 10.2210/pdb7p6j/pdb
PDB entry 7p6j coloured by chain, front view.
PDB entry 7p6j coloured by chain, side view.
PDB entry 7p6j coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Highlighting the factors governing transglycosylation in the GH5_5 endo-1,4-β-glucanase RBcel1.
Collet L, Vander Wauven C, Oudjama Y, Galleni M, Dutoit R
Acta Crystallogr D Struct Biol 78 278-289 (2022)
PMID: 35234142

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
Glycoside hydrolase family 5 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 321 amino acids
Theoretical weight: 36.37 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: C1JI15 (Residues: 31-351; Coverage: 100%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 88.56Å b: 90.54Å c: 89.68Å
α: 90° β: 118.77° γ: 90°
R-values:
R R work R free
0.174 0.172 0.202
Expression system: Escherichia coli MC1061

Quick links

Citations

1 citations in other articles

The Role of a Loop in the Non-catalytic Domain B on the Hydrolysis/Transglycosylation Specificity of the 4-α-Glucanotransferase from Thermotoga maritima.
Llopiz et al. (2023)

2 mentions without citation

Molecular basis for the initiation of DNA primer synthesis.
Li et al. (2022)
1 more