› 7p6x

Electron Microscopy
4.1Å resolution

Cryo-Em structure of the hexameric RUVBL1-RUVBL2 in complex with ZNHIT2

Released:
DOI: 10.2210/pdb7p6x/pdb
PDB entry 7p6x coloured by chain, front view.
PDB entry 7p6x coloured by chain, side view.
PDB entry 7p6x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8.
Serna M, González-Corpas A, Cabezudo S, López-Perrote A, Degliesposti G, Zarzuela E, Skehel JM, Muñoz J, Llorca O
OpenAccess logo Nucleic Acids Res 50 1128-1146 (2022)
PMID: 34951455
Related structures: EMD-13233

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195084 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RuvB-like 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 456 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y265 (Residues: 1-456; Coverage: 100%)
Gene names: INO80H, NMP238, RUVBL1, TIP49, TIP49A
Sequence domains:
RuvB-like 2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 463 amino acids
Theoretical weight: 51.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y230 (Residues: 1-463; Coverage: 100%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-13233
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Role of Hsp90-R2TP in Macromolecular Complex Assembly and Stabilization.
Lynham et al. (2022)
1 other article

1 mention without citation

CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8.
Serna et al. (2022)