› 7p7e

Electron Microscopy
2.7Å resolution

Complex I from E. coli, DDM/LMNG-purified, Apo, Resting state

Released:
DOI: 10.2210/pdb7p7e/pdb
PDB entry 7p7e coloured by chain, front view.
PDB entry 7p7e coloured by chain, side view.
PDB entry 7p7e coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
A universal coupling mechanism of respiratory complex I.
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022)
PMID: 36104567
Related structures: EMD-13236

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142708 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
NADH-quinone oxidoreductase subunit F Chain: F
Molecule details ›
Chain: F
Length: 442 amino acids
Theoretical weight: 48.88 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31979 (Residues: 1-442; Coverage: 99%)
Gene names: JW2279, b2284, nuoF
Sequence domains:
NADH-quinone oxidoreductase subunit E Chain: E
Molecule details ›
Chain: E
Length: 156 amino acids
Theoretical weight: 17.39 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFD1 (Residues: 11-166; Coverage: 94%)
Gene names: JW2280, b2285, nuoE
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase subunit G Chain: G
Molecule details ›
Chain: G
Length: 905 amino acids
Theoretical weight: 99.96 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33602 (Residues: 2-906; Coverage: 100%)
Gene names: JW2278, b2283, nuoG
Sequence domains:
NADH-quinone oxidoreductase subunit C/D Chain: C
Molecule details ›
Chain: C
Length: 600 amino acids
Theoretical weight: 68.81 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33599 (Residues: 1-596; Coverage: 100%)
Gene names: JW5375, b2286, nuoC, nuoCD, nuoD
Sequence domains:
NADH-quinone oxidoreductase subunit B Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 25.08 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFC7 (Residues: 1-220; Coverage: 100%)
Gene names: JW5875, b2287, nuoB
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
NADH-quinone oxidoreductase subunit I Chain: I
Molecule details ›
Chain: I
Length: 145 amino acids
Theoretical weight: 16.46 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFD6 (Residues: 36-180; Coverage: 81%)
Gene names: JW2276, b2281, nuoI
Sequence domains: 4Fe-4S binding domain
NADH-quinone oxidoreductase subunit H Chain: H
Molecule details ›
Chain: H
Length: 325 amino acids
Theoretical weight: 36.24 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFD4 (Residues: 1-325; Coverage: 100%)
Gene names: JW2277, b2282, nuoH
Sequence domains: NADH dehydrogenase
NADH-quinone oxidoreductase subunit A Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.47 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFC3 (Residues: 1-147; Coverage: 100%)
Gene names: JW2283, b2288, nuoA
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
NADH-quinone oxidoreductase subunit L Chain: L
Molecule details ›
Chain: L
Length: 613 amino acids
Theoretical weight: 66.51 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33607 (Residues: 1-613; Coverage: 100%)
Gene names: JW2273, b2278, nuoL
Sequence domains:
NADH-quinone oxidoreductase subunit M Chain: M
Molecule details ›
Chain: M
Length: 504 amino acids
Theoretical weight: 56 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFE8 (Residues: 1-504; Coverage: 99%)
Gene names: JW2272, b2277, nuoM
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit N Chain: N
Molecule details ›
Chain: N
Length: 485 amino acids
Theoretical weight: 52.07 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFF0 (Residues: 1-485; Coverage: 100%)
Gene names: JW2271, b2276, nuoN
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit K Chain: K
Molecule details ›
Chain: K
Length: 100 amino acids
Theoretical weight: 10.85 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFE4 (Residues: 1-100; Coverage: 100%)
Gene names: JW2274, b2279, nuoK
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-quinone oxidoreductase subunit J Chain: J
Molecule details ›
Chain: J
Length: 184 amino acids
Theoretical weight: 19.89 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFE0 (Residues: 1-184; Coverage: 100%)
Gene names: JW2275, b2280, nuoJ
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6

Ligands and Environments

6 bound ligands:
Ligand SF4 7 x SF4
Ligand FMN 1 x FMN
Ligand FES 2 x FES
Ligand CA 1 x CA
Ligand 3PE 9 x 3PE
Ligand LFA 3 x LFA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-13236

Quick links

Citations

4 review citations

An evolving view of complex II-noncanonical complexes, megacomplexes, respiration, signaling, and beyond.
Iverson et al. (2023)
3 more