7p7l

Electron Microscopy
3Å resolution

Complex I from E. coli, DDM/LMNG-purified, with NADH and FMN, Open state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-142708 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
NADH-quinone oxidoreductase subunit F Chain: F
Molecule details ›
Chain: F
Length: 439 amino acids
Theoretical weight: 48.51 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31979 (Residues: 4-442; Coverage: 99%)
Gene names: JW2279, b2284, nuoF
Sequence domains:
NADH-quinone oxidoreductase subunit E Chain: E
Molecule details ›
Chain: E
Length: 156 amino acids
Theoretical weight: 17.39 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFD1 (Residues: 11-166; Coverage: 94%)
Gene names: JW2280, b2285, nuoE
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase subunit C/D Chain: C
NADH-quinone oxidoreductase subunit B Chain: B
NADH-quinone oxidoreductase subunit I Chain: I
NADH-quinone oxidoreductase subunit H Chain: H
NADH-quinone oxidoreductase subunit A Chain: A
NADH-quinone oxidoreductase subunit L Chain: L
NADH-quinone oxidoreductase subunit M Chain: M
NADH-quinone oxidoreductase subunit N Chain: N
NADH-quinone oxidoreductase subunit K Chain: K
NADH-quinone oxidoreductase subunit J Chain: J

Ligands and Environments

No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution:
Relevant EMDB volumes: EMD-13239