› 7p97

X-ray diffraction
2.35Å resolution

Structure of 3-phospho-D-glycerate guanylyltransferase with product 3-GPPG bound

Released:
DOI: 10.2210/pdb7p97/pdb
PDB entry 7p97 coloured by chain, front view.
PDB entry 7p97 coloured by chain, side view.
PDB entry 7p97 coloured by chain, top view.
Source organism: Mycetohabitans rhizoxinica HKI 454
Primary publication:
Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F420 and Its Derivative 3PG-F420.
Hasan M, Schulze S, Berndt L, Palm GJ, Braga D, Richter I, Last D, Lammers M, Lackner G
OpenAccess logo mBio e0350121 (2022)
PMID: 35038903

Function and Biology Details

Reaction catalysed: 
3-phospho-D-glycerate + GTP = 3-(D-glyceryl)-diphospho-5'-guanosine + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phospho-D-glycerate guanylyltransferase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 243 amino acids
Theoretical weight: 25.91 KDa
Source organism: Mycetohabitans rhizoxinica HKI 454
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: E5ASS2 (Residues: 6-220; Coverage: 97%)
Gene names: RBRH_02550, cofC
Sequence domains: Guanylyl transferase CofC like

Ligands and Environments

3 bound ligands:
Ligand 6WI 2 x 6WI
Ligand MG 4 x MG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3221
Unit cell:
a: 57.09Å b: 57.09Å c: 228.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.186 0.238
Expression system: Escherichia coli BL21

Quick links

Citations

1 mention without citation

Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F420 and Its Derivative 3PG-F420.
Hasan et al. (2022)