› 7paz

X-ray diffraction
2Å resolution

REDUCED MUTANT P80I PSEUDOAZURIN FROM A. FAECALIS

Released:
DOI: 10.2210/pdb7paz/pdb
PDB entry 7paz coloured by chain, front view.
PDB entry 7paz coloured by chain, side view.
PDB entry 7paz coloured by chain, top view.
Source organism: Alcaligenes faecalis
Primary publication:
Site-directed mutants of pseudoazurin: explanation of increased redox potentials from X-ray structures and from calculation of redox potential differences.
Libeu CA, Kukimoto M, Nishiyama M, Horinouchi S, Adman ET
Biochemistry 36 13160-79 (1997)
PMID: 9341204

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-528266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudoazurin Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.4 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: P04377 (Residues: 24-146; Coverage: 100%)
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P65
Unit cell:
a: 50.79Å b: 50.79Å c: 98.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 not available not available
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
5 more