› 7php

Electron Microscopy
3.47Å resolution

Structure of Multidrug and Toxin Compound Extrusion (MATE) transporter NorM by NabFab-fiducial assisted cryo-EM

Released:
DOI: 10.2210/pdb7php/pdb
PDB entry 7php coloured by chain, front view.
PDB entry 7php coloured by chain, side view.
PDB entry 7php coloured by chain, top view.
Source organisms:
Primary publication:
Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34782475
Related structures: EMD-13424

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212776 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Multidrug resistance protein NorM Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 52.46 KDa
Source organism: Vibrio cholerae RC385
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KRU4 (Residues: 1-457; Coverage: 100%)
Gene names: VC_1540, norM, vcmA
Sequence domains: MatE
NabFab HC Chain: H
Molecule details ›
Chain: H
Length: 239 amino acids
Theoretical weight: 25.68 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
NabFab LC Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
NorM-Nb17_4 Chain: N
Molecule details ›
Chain: N
Length: 132 amino acids
Theoretical weight: 14.36 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Anti-Fab nanobody Chain: K
Molecule details ›
Chain: K
Length: 123 amino acids
Theoretical weight: 13.39 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.47Å
Relevant EMDB volumes: EMD-13424
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Putting on molecular weight: Enabling cryo-EM structure determination of sub-100-kDa proteins.
Wentinck et al. (2022)
1 more

3 mentions without citation

Functional Roles of the Conserved Amino Acid Sequence Motif C, the Antiporter Motif, in Membrane Transporters of the Major Facilitator Superfamily.
Varela et al. (2023)
2 more