› 7phq

Electron Microscopy
8.45Å resolution

Structure of homo-dimeric Staphylococcus capitis divalent metal ion transporter (DMT) by NabFab-fiducial assisted cryo-EM

Released:
DOI: 10.2210/pdb7phq/pdb
PDB entry 7phq coloured by chain, front view.
PDB entry 7phq coloured by chain, side view.
PDB entry 7phq coloured by chain, top view.
Source organisms:
Primary publication:
Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34782475
Related structures: EMD-13426

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207460 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
NabFab HC Chains: A, H
Molecule details ›
Chains: A, H
Length: 239 amino acids
Theoretical weight: 25.68 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
NabFab LC Chains: C, L
Molecule details ›
Chains: C, L
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Divalent metal cation transporter MntH Chains: B, D
Molecule details ›
Chains: B, D
Length: 427 amino acids
Theoretical weight: 47.05 KDa
Source organism: Staphylococcus capitis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4U9TNH6 (Residues: 41-41, 43-448; Coverage: 91%)
Gene names: NCTC11045_01736, mntH
Sequence domains: Natural resistance-associated macrophage protein-like
DMT-Nb16_4 Chains: E, N
Molecule details ›
Chains: E, N
Length: 130 amino acids
Theoretical weight: 14.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Anti-Fab nanobody Chains: F, K
Molecule details ›
Chains: F, K
Length: 123 amino acids
Theoretical weight: 13.39 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.45Å
Relevant EMDB volumes: EMD-13426
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Functional Roles of the Conserved Amino Acid Sequence Motif C, the Antiporter Motif, in Membrane Transporters of the Major Facilitator Superfamily.
Varela et al. (2023)
2 more

1 mention without citation

A systematic review of neuropsychological and psychiatric sequalae of COVID-19: implications for treatment.
Vanderlind et al. (2021)