› 7pik

Electron Microscopy
2.68Å resolution

Cryo-EM structure of E. coli TnsB in complex with right end fragment of Tn7 transposon

Released:
DOI: 10.2210/pdb7pik/pdb
PDB entry 7pik coloured by chain, front view.
PDB entry 7pik coloured by chain, side view.
PDB entry 7pik coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of transposon end recognition explains central features of Tn7 transposition systems.
Kaczmarska Z, Czarnocki-Cieciura M, Górecka-Minakowska KM, Wingo RJ, Jackiewicz J, Zajko W, Poznański JT, Rawski M, Grant T, Peters JE, Nowotny M
OpenAccess logo Mol Cell (2022)
PMID: 35654042
Related structures: EMD-13439

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121817 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposon Tn7 transposition protein TnsB Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 703 amino acids
Theoretical weight: 81.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13989 (Residues: 1-702; Coverage: 100%)
Gene name: tnsB
Right end fragment of Tn7 transposon Chain: K
Molecule details ›
Chain: K
Length: 70 nucleotides
Theoretical weight: 21.47 KDa
Source organism: Escherichia coli
Expression system: Not provided
Right end fragment of Tn7 transposon Chain: L
Molecule details ›
Chain: L
Length: 70 nucleotides
Theoretical weight: 21.68 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.68Å
Relevant EMDB volumes: EMD-13439
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Genetically engineered bacterium: Principles, practices, and prospects.
Liu et al. (2022)
9 other articles

3 mentions without citation

Structural basis of transposon end recognition explains central features of Tn7 transposition systems.
Kaczmarska et al. (2022)
2 more