› 7pk4

X-ray diffraction
1.92Å resolution

Function and Biology Details

Reaction catalysed: 
The recombinant enzyme hydrolyzes proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102335 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cathepsin L2 Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O60911 (Residues: 113-334; Coverage: 70%)
Gene names: CATL2, CTSL2, CTSU, CTSV, UNQ268/PRO305
Sequence domains: Papain family cysteine protease
Cystatin domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 12.75 KDa
Source organism: Ixodes ricinus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K8RMA7 (Residues: 19-133; Coverage: 100%)
Sequence domains: Cystatin domain

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand EDO 6 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 117.488Å b: 45.322Å c: 58.52Å
α: 90° β: 96.89° γ: 90°
R-values:
R R work R free
0.188 0.186 0.226
Expression systems:
  • Komagataella pastoris
  • Escherichia coli BL21(DE3)

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