› 7pqe

Electron Microscopy
3.7Å resolution

Structure of SidJ/CaM bound to SdeA in post-catalysis state

Released:
DOI: 10.2210/pdb7pqe/pdb
PDB entry 7pqe coloured by chain, front view.
PDB entry 7pqe coloured by chain, side view.
PDB entry 7pqe coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for protein glutamylation by the Legionella pseudokinase SidJ.
Adams M, Sharma R, Colby T, Weis F, Matic I, Bhogaraju S
OpenAccess logo Nat Commun 12 6174 (2021)
PMID: 34702826
Related structures: EMD-13591

Function and Biology Details

Reaction catalysed: 
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-144001 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitinating/deubiquitinating enzyme SdeA Chain: A
Molecule details ›
Chain: A
Length: 979 amino acids
Theoretical weight: 110.88 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZTK4 (Residues: 231-1190; Coverage: 64%)
Gene names: lpg2157, sdeA
Sequence domains:
Calmodulin-dependent glutamylase SidJ Chain: C
Molecule details ›
Chain: C
Length: 794 amino acids
Theoretical weight: 91.83 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZTK6 (Residues: 99-873; Coverage: 89%)
Gene names: lpg2155, sidJ
Calmodulin-2 Chain: B
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 19.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP24 (Residues: 1-149; Coverage: 100%)
Gene names: CALM2, CAM2, CAMB
Sequence domains: EF-hand domain pair

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-13591
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Ubiquitin-targeted bacterial effectors: rule breakers of the ubiquitin system.
Roberts et al. (2023)
3 more

1 mention without citation

Exaptation of Inactivated Host Enzymes for Structural Roles in Orthopoxviruses and Novel Folds of Virus Proteins Revealed by Protein Structure Modeling.
Mutz et al. (2023)