7prg

Hybrid

Joint X-ray/neutron room temperature structure of perdeuterated LecB lectin in complex with perdeuterated fucose

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-100417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 11.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A069Q9V4 (Residues: 1-115; Coverage: 100%)
Gene names: CAZ10_21840, DT376_00595, GUL26_03290, IPC1295_29365, PAERUG_P19_London_7_VIM_2_05_10_00588
Sequence domains: Fucose-binding lectin II (PA-IIL)

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Experimental Method: X-ray diffraction, Neutron Diffraction
X-ray source: Xenocs GeniX 3D Cu HF, ILL BEAMLINE LADI III
Spacegroup: P21
Unit cell:
a: 52.9Å b: 73.87Å c: 55.003Å
α: 90° β: 94.58° γ: 90°
R-values:
R R work R free
0.106 0.104 0.142
Expression system: Escherichia coli BL21(DE3)