› 7pt7

Electron Microscopy
3.8Å resolution

Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I

Released:
DOI: 10.2210/pdb7pt7/pdb
PDB entry 7pt7 coloured by chain, front view.
PDB entry 7pt7 coloured by chain, side view.
PDB entry 7pt7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
Saleh A, Noguchi Y, Aramayo R, Ivanova ME, Stevens KM, Montoya A, Sunidhi S, Carranza NL, Skwark MJ, Speck C
OpenAccess logo Nat Commun 13 2915 (2022)
PMID: 35614055
Related structures: EMD-13620

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-138978 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Undefined Mcm4 flexible N-terminal tail Chain: 1
Molecule details ›
Chain: 1
Length: 4 amino acids
Theoretical weight: 358 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
DNA replication licensing factor MCM2 Chains: 2, B
Molecule details ›
Chains: 2, B
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chains: 3, C
Molecule details ›
Chains: 3, C
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chains: 4, D
Molecule details ›
Chains: 4, D
Length: 933 amino acids
Theoretical weight: 105.14 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 1-933; Coverage: 100%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
Minichromosome maintenance protein 5 Chains: 5, E
Molecule details ›
Chains: 5, E
Length: 775 amino acids
Theoretical weight: 86.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29496 (Residues: 1-775; Coverage: 100%)
Gene names: CDC46, L9328.1, MCM5, YLR274W
Sequence domains:
DNA replication licensing factor MCM6 Chains: 6, F
Molecule details ›
Chains: 6, F
Length: 1017 amino acids
Theoretical weight: 113.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 1-1017; Coverage: 100%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM7 Chains: 7, G
Molecule details ›
Chains: 7, G
Length: 845 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-845; Coverage: 100%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
Cell division control protein 7 Chain: 8
Molecule details ›
Chain: 8
Length: 507 amino acids
Theoretical weight: 58.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P06243 (Residues: 1-507; Coverage: 100%)
Gene names: CDC7, D2855, OAF2, YDL017W
Sequence domains: Protein kinase domain
DDK kinase regulatory subunit DBF4 Chain: 9
Molecule details ›
Chain: 9
Length: 704 amino acids
Theoretical weight: 80.82 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32325 (Residues: 1-704; Coverage: 100%)
Gene names: D4205, DBF4, DNA52, YD9609.07C, YDR052C
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 13 x ADP
Ligand MG 14 x MG
Ligand ZN 12 x ZN
Ligand BEF 7 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-13620
Expression system: Saccharomyces cerevisiae

Quick links

Citations

4 review citations

MCM2 in human cancer: functions, mechanisms, and clinical significance.
Sun et al. (2022)
3 more