› 7pt7

Electron Microscopy
3.8Å resolution

Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I

Released:
DOI: 10.2210/pdb7pt7/pdb
PDB entry 7pt7 coloured by chain, front view.
PDB entry 7pt7 coloured by chain, side view.
PDB entry 7pt7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
Saleh A, Noguchi Y, Aramayo R, Ivanova ME, Stevens KM, Montoya A, Sunidhi S, Carranza NL, Skwark MJ, Speck C
OpenAccess logo Nat Commun 13 2915 (2022)
PMID: 35614055
Related structures: EMD-13620

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-138978 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Undefined Mcm4 flexible N-terminal tail Chain: 1
Molecule details ›
Chain: 1
Length: 4 amino acids
Theoretical weight: 358 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
DNA replication licensing factor MCM2 Chains: 2, B
Molecule details ›
Chains: 2, B
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chains: 3, C
DNA replication licensing factor MCM4 Chains: 4, D
Minichromosome maintenance protein 5 Chains: 5, E
DNA replication licensing factor MCM6 Chains: 6, F
DNA replication licensing factor MCM7 Chains: 7, G
Cell division control protein 7 Chain: 8
DDK kinase regulatory subunit DBF4 Chain: 9

Ligands and Environments

4 bound ligands:
Ligand ADP 13 x ADP
Ligand MG 14 x MG
Ligand ZN 12 x ZN
Ligand BEF 7 x BEF
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-13620
Expression system: Saccharomyces cerevisiae

Quick links

Citations

4 review citations

MCM2 in human cancer: functions, mechanisms, and clinical significance.
Sun et al. (2022)
3 more