› 7ptf

X-ray diffraction
1.32Å resolution

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 221 amino acids
Theoretical weight: 24.54 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I7C2 (Residues: 1-221; Coverage: 27%)
Gene names: PA0004, gyrB
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Ligands and Environments

3 bound ligands:
Ligand NOV 3 x NOV
Ligand MRD 2 x MRD
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 62.657Å b: 47.391Å c: 110.626Å
α: 90° β: 99.49° γ: 90°
R-values:
R R work R free
0.15 0.148 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Developments in Non-Intercalating Bacterial Topoisomerase Inhibitors: Allosteric and ATPase Inhibitors of DNA Gyrase and Topoisomerase IV.
Grossman et al. (2023)
4 other articles

2 mentions without citation

Discovery and Hit-to-Lead Optimization of Benzothiazole Scaffold-Based DNA Gyrase Inhibitors with Potent Activity against Acinetobacter baumannii and Pseudomonas aeruginosa.
Cotman et al. (2023)
1 more