› 7pup

X-ray diffraction
1.91Å resolution

INOSITOL 1,3,4-TRISPHOSPHATE 5/6-KINASE 4 from Arabidopsis thaliana (AtITPK4) in complex with ATP

Released:
DOI: 10.2210/pdb7pup/pdb
PDB entry 7pup coloured by chain, front view.
PDB entry 7pup coloured by chain, side view.
PDB entry 7pup coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Diversification in the inositol tris/tetrakisphosphate kinase (ITPK) family: crystal structure and enzymology of the outlier AtITPK4.
Whitfield HL, He S, Gu Y, Sprigg C, Kuo HF, Chiou TJ, Riley AM, Potter BVL, Hemmings AM, Brearley CA
OpenAccess logo Biochem J 480 433-453 (2023)
PMID: 36896917

Function and Biology Details

Reaction catalysed: 
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol 1,3,4-trisphosphate 5/6-kinase 4 Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 54.4 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O80568 (Residues: 1-488; Coverage: 100%)
Gene names: At2g43980, F6E13.11, ITPK4
Sequence domains: Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp domain

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 45.618Å b: 61.807Å c: 176.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.241
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

ITPKA phosphorylates PYCR1 and promotes the progression of glioma.
Luo et al. (2024)

2 mentions without citation

Diversification in the inositol tris/tetrakisphosphate kinase (ITPK) family: crystal structure and enzymology of the outlier AtITPK4.
Whitfield et al. (2023)
1 more