› 7put

X-ray diffraction
2.8Å resolution

Crystal structure of Thioredoxin Reductase from Brugia Malayi in complex with NADP(H)

Released:
DOI: 10.2210/pdb7put/pdb
PDB entry 7put coloured by chain, front view.
PDB entry 7put coloured by chain, side view.
PDB entry 7put coloured by chain, top view.
Source organism: Brugia malayi
Primary publication:
Biochemical and structural characterizations of thioredoxin reductase selenoproteins of the parasitic filarial nematodes Brugia malayi and Onchocerca volvulus.
Fata F, Gencheva R, Cheng Q, Lullo R, Ardini M, Silvestri I, Gabriele F, Ippoliti R, Bulman CA, Sakanari JA, Williams DL, Arnér ESJ, Angelucci F
OpenAccess logo Redox Biol 51 102278 (2022)
PMID: 35276442

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103484 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
thioredoxin-disulfide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 598 amino acids
Theoretical weight: 66.12 KDa
Source organism: Brugia malayi
Expression system: synthetic Escherichia coli C321.deltaA
UniProt:
  • Canonical: A0A1I9G0T9 (Residues: 116-713; Coverage: 84%)
Gene names: BM_Bm2025, Bm2025, Bma-trxr-1, bma-trxr-1
Sequence domains:
thioredoxin-disulfide reductase Chain: C
Molecule details ›
Chain: C
Length: 598 amino acids
Theoretical weight: 66.09 KDa
Source organism: Brugia malayi
Expression system: synthetic Escherichia coli C321.deltaA
UniProt:
  • Canonical: A0A1I9G0T9 (Residues: 116-713; Coverage: 84%)
Gene names: BM_Bm2025, Bm2025, Bma-trxr-1, bma-trxr-1
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 3 x FAD
2 bound ligands:
Ligand MPD 7 x MPD
Ligand ATR 1 x ATR
1 modified residue:
Ligand 2CO 2 x 2CO

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: C2221
Unit cell:
a: 147.39Å b: 260.287Å c: 130.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.229
Expression system: synthetic Escherichia coli C321.deltaA

Quick links

Citations

3 citation in other articles

Non-covalent inhibitors of thioredoxin glutathione reductase with schistosomicidal activity in vivo.
Petukhova et al. (2023)
2 more

1 mention without citation

Biochemical and structural characterizations of thioredoxin reductase selenoproteins of the parasitic filarial nematodes Brugia malayi and Onchocerca volvulus.
Fata et al. (2022)