› 7px3

Electron Microscopy
3.05Å resolution

Structure of U5 snRNP assembly and recycling factor TSSC4 in complex with BRR2 and Jab1 domain of PRPF8

Released:
DOI: 10.2210/pdb7px3/pdb
PDB entry 7px3 coloured by chain, front view.
PDB entry 7px3 coloured by chain, side view.
PDB entry 7px3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The intrinsically disordered TSSC4 protein acts as a helicase inhibitor, placeholder and multi-interaction coordinator during snRNP assembly and recycling.
Bergfort A, Hilal T, Kuropka B, Ilik İA, Weber G, Aktaş T, Freund C, Wahl MC
OpenAccess logo Nucleic Acids Res 50 2938-2958 (2022)
PMID: 35188580
Related structures: EMD-13690

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-131093 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
U5 small nuclear ribonucleoprotein 200 kDa helicase Chain: B
Molecule details ›
Chain: B
Length: 1747 amino acids
Theoretical weight: 199.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75643 (Residues: 394-2136; Coverage: 82%)
Gene names: ASCC3L1, BRR2, HELIC2, KIAA0788, SNRNP200
Sequence domains:
Pre-mRNA-processing-splicing factor 8 Chain: J
Molecule details ›
Chain: J
Length: 263 amino acids
Theoretical weight: 30.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6P2Q9 (Residues: 2064-2320; Coverage: 11%)
Gene names: PRPC8, PRPF8
Sequence domains:
U5 small nuclear ribonucleoprotein TSSC4 Chain: T
Molecule details ›
Chain: T
Length: 329 amino acids
Theoretical weight: 34.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5U2 (Residues: 1-329; Coverage: 100%)
Gene name: TSSC4
Sequence domains: Tumour suppressing sub-chromosomal transferable candidate 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.05Å
Relevant EMDB volumes: EMD-13690
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Structural and functional investigation of the human snRNP assembly factor AAR2 in complex with the RNase H-like domain of PRPF8.
Preussner et al. (2022)
4 more

2 mentions without citation

The intrinsically disordered TSSC4 protein acts as a helicase inhibitor, placeholder and multi-interaction coordinator during snRNP assembly and recycling.
Bergfort et al. (2022)
1 more