› 7pxa

Electron Microscopy
2.8Å resolution

Open-gate mycobacterium 20S CP proteasome in complex MPA - global 3D refinement

Released:
DOI: 10.2210/pdb7pxa/pdb
PDB entry 7pxa coloured by chain, front view.
PDB entry 7pxa coloured by chain, side view.
PDB entry 7pxa coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural basis of prokaryotic ubiquitin-like protein engagement and translocation by the mycobacterial Mpa-proteasome complex.
Kavalchuk M, Jomaa A, Müller AU, Weber-Ban E
OpenAccess logo Nat Commun 13 276 (2022)
PMID: 35022401
Related structures: EMD-13695

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161589 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasome subunit alpha Chains: 0, 2, 4, 6, 8, I, K, O, Q, T, X, Z, d, f
Molecule details ›
Chains: 0, 2, 4, 6, 8, I, K, O, Q, T, X, Z, d, f
Length: 248 amino acids
Theoretical weight: 26.91 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 1-248; Coverage: 100%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Proteasome-associated ATPase Chains: 1, A, B, C, D, E, F
Molecule details ›
Chains: 1, A, B, C, D, E, F
Length: 609 amino acids
Theoretical weight: 67.49 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQN5 (Residues: 1-609; Coverage: 100%)
Gene names: MTCY261.11c, Rv2115c, mpa
Sequence domains:
Proteasome subunit beta Chains: H, J, L, M, N, P, R, S, U, V, W, Y, a, b
Molecule details ›
Chains: H, J, L, M, N, P, R, S, U, V, W, Y, a, b
Length: 291 amino acids
Theoretical weight: 30.33 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 1-291; Coverage: 100%)
Gene names: Rv2110c, prcB
Sequence domains: Proteasome subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-13695
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

AAA+ protease-adaptor structures reveal altered conformations and ring specialization.
Kim et al. (2022)
4 more