› 7py6

Electron Microscopy
4.1Å resolution

CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA and NusG elongation complex in less-swiveled conformation)

Released:
DOI: 10.2210/pdb7py6/pdb
PDB entry 7py6 coloured by chain, front view.
PDB entry 7py6 coloured by chain, side view.
PDB entry 7py6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Transcription factors modulate RNA polymerase conformational equilibrium.
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-André C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun 13 1546 (2022)
PMID: 35318334
Related structures: EMD-13714

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-121832 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
Transcription termination/antitermination protein NusG Chain: G
Molecule details ›
Chain: G
Length: 181 amino acids
Theoretical weight: 20.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFG0 (Residues: 1-181; Coverage: 100%)
Gene names: JW3945, b3982, nusG
Sequence domains:
Transcription termination/antitermination protein NusA Chain: F
Molecule details ›
Chain: F
Length: 495 amino acids
Theoretical weight: 54.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFF6 (Residues: 1-495; Coverage: 100%)
Gene names: JW3138, b3169, nusA
Sequence domains:
ntDNA Chain: N
Molecule details ›
Chain: N
Length: 39 nucleotides
Theoretical weight: 12.06 KDa
tDNA Chain: T
Molecule details ›
Chain: T
Length: 39 nucleotides
Theoretical weight: 11.9 KDa
RNA Chain: R
Molecule details ›
Chain: R
Length: 14 nucleotides
Theoretical weight: 4.53 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-13714
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Structural basis for intrinsic transcription termination.
You et al. (2023)
7 more