› 7q16

X-ray diffraction
2.36Å resolution

Human 14-3-3 zeta fused to the BAD peptide including phosphoserine-74

Released:
DOI: 10.2210/pdb7q16/pdb
Model geometry
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PDB entry 7q16 coloured by chain, front view.
PDB entry 7q16 coloured by chain, side view.
PDB entry 7q16 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human 14-3-3ζ complexed with the noncanonical phosphopeptide from proapoptotic BAD.
Sluchanko NN, Tugaeva KV, Gushchin I, Remeeva A, Kovalev K, Cooley RB
Biochem Biophys Res Commun 583 100-105 (2021)
PMID: 34735870

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14-3-3 protein zeta/delta; Bcl2-associated agonist of cell death Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63104 (Residues: 1-229; Coverage: 94%)
  • Canonical: Q92934 (Residues: 71-81; Coverage: 7%)
Gene names: BAD, BBC6, BCL2L8, YWHAZ
Sequence domains: 14-3-3 protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 85.698Å b: 112.147Å c: 75.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.241
Expression system: Escherichia coli

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Citations

1 review citation

Is 14-3-3 the Combination to Unlock New Pathways to Improve Metabolic Homeostasis and β-Cell Function?
Rial et al. (2023)

2 mentions without citation

Vertical pathway inhibition of receptor tyrosine kinases and BAD with synergistic efficacy in triple negative breast cancer.
Tan et al. (2024)
1 more

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