› 7q1f

X-ray diffraction
2.35Å resolution

CPAP:TUBULIN:IE5 ALPHAREP COMPLEX

Released:
DOI: 10.2210/pdb7q1f/pdb
Model geometry
Fit model/data
PDB entry 7q1f coloured by chain, front view.
PDB entry 7q1f coloured by chain, side view.
PDB entry 7q1f coloured by chain, top view.
Source organisms:
Primary publication:
Structural convergence for tubulin binding of CPAP and vinca domain microtubule inhibitors.
Campanacci V, Urvoas A, Ammar Khodja L, Aumont-Nicaise M, Noiray M, Lachkar S, Curmi PA, Minard P, Gigant B
OpenAccess logo Proc Natl Acad Sci U S A 119 e2120098119 (2022)
PMID: 35507869

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168645 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chains: A, R
Molecule details ›
Chains: A, R
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
Tubulin beta chain Chains: B, S
Molecule details ›
Chains: B, S
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Ovis aries
IE5 ALPHAREP Chains: C, D, T, U
Centrosomal P4.1-associated protein Chains: P, V

Ligands and Environments

6 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand SO4 2 x SO4
Ligand GDP 2 x GDP
Ligand MES 4 x MES
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 89.088Å b: 215.864Å c: 95.536Å
α: 90° β: 109.61° γ: 90°
R-values:
R R work R free
0.19 0.188 0.22
Expression system: Escherichia coli

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Citations

1 review citation

Computational Approaches to the Rational Design of Tubulin-Targeting Agents.
Pérez-Peña et al. (2023)