› 7q34

X-ray diffraction
2.6Å resolution

Crystal structure of the multidrug binding transcriptional regulator LmrR in complex squaraine dye

Released:
DOI: 10.2210/pdb7q34/pdb
PDB entry 7q34 coloured by chain, front view.
PDB entry 7q34 coloured by chain, side view.
PDB entry 7q34 coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Primary publication:
Designing Artificial Fluorescent Proteins: Squaraine-LmrR Biophosphors for High Performance Deep-Red Biohybrid Light-Emitting Diodes
Ferrara S, Mejias SH, Liutkus M, Renno G, Stella F, Kociolek I, Fuenzalida-Werner JP, Barolo C, Coto PB, Cortajarena AL, Costa RD
Adv Funct Mater 32 2111381- (2022)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription regulator PadR N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 132 amino acids
Theoretical weight: 14.89 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0A7SZD7 (Residues: 2-111; Coverage: 95%)
Gene names: CYU10_001323, EFK44_05110, JCM5805K_2657, KF282_0527, LL14B4_01620, LL223_0393, LL229_0409, LL275_0346, LLUC06_0422, LLUC11_0290, LMG8520_0357, LMG8526_0070, LMG9449_1101, Li1_2044, N42_0245
Sequence domains: Transcriptional regulator PadR-like family

Ligands and Environments

2 bound ligands:
Ligand NI 4 x NI
Ligand 8TF 2 x 8TF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 44.658Å b: 68.019Å c: 200.858Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.241 0.317
Expression system: Escherichia coli BL21(DE3)

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