› 7q3n

Electron Microscopy
7.4Å resolution

Cryo-EM of the complex between human uromodulin (UMOD)/Tamm-Horsfall protein (THP) and the FimH lectin domain from uropathogenic E. coli

Released:
DOI: 10.2210/pdb7q3n/pdb
PDB entry 7q3n coloured by chain, front view.
PDB entry 7q3n coloured by chain, side view.
PDB entry 7q3n coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.
Stsiapanava A, Xu C, Nishio S, Han L, Yamakawa N, Carroni M, Tunyasuvunakool K, Jumper J, de Sanctis D, Wu B, Jovine L
OpenAccess logo Nat Struct Mol Biol 29 190-193 (2022)
PMID: 35273390
Related structures: EMD-13794

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139836 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Uromodulin Chain: U
Molecule details ›
Chain: U
Length: 563 amino acids
Theoretical weight: 61.5 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07911 (Residues: 25-587; Coverage: 91%)
Gene name: UMOD
Sequence domains:
Type 1 fimbiral adhesin FimH Chain: F
Molecule details ›
Chain: F
Length: 172 amino acids
Theoretical weight: 18.67 KDa
Source organism: Escherichia coli UTI89
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1R2J4 (Residues: 22-180; Coverage: 57%)
Gene names: UTI89_C5017, fimH
Sequence domains: FimH, mannose binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.4Å
Relevant EMDB volumes: EMD-13794
Expression system: Escherichia coli

Quick links

Citations

2 review citations

AlphaFold2 and its applications in the fields of biology and medicine.
Yang et al. (2023)
1 more

1 mention without citation

Structural insights into a cooperative switch between one and two FimH bacterial adhesins binding pauci- and high-mannose type N-glycan receptors.
Krammer et al. (2023)