› 7q4i

X-ray diffraction
2.4Å resolution

Crystal structure of DmC1GalT1 in complex with UDP-Mn2+ and the APD-TGalNAc-RP

Released:
DOI: 10.2210/pdb7q4i/pdb
PDB entry 7q4i coloured by chain, front view.
PDB entry 7q4i coloured by chain, side view.
PDB entry 7q4i coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the synthesis of the core 1 structure by C1GalT1.
González-Ramírez AM, Grosso AS, Yang Z, Compañón I, Coelho H, Narimatsu Y, Clausen H, Marcelo F, Corzana F, Hurtado-Guerrero R
OpenAccess logo Nat Commun 13 2398 (2022)
PMID: 35504880

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147571 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 36.27 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K237 (Residues: 73-388; Coverage: 81%)
Gene names: C1GalTA, CG9520
Sequence domains: Fringe-like
Mucin-1 subunit alpha Chains: F, G
Molecule details ›
Chains: F, G
Length: 6 amino acids
Theoretical weight: 656 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P15941 (Residues: 921-926; Coverage: 1%)
Gene names: MUC1, PUM

Ligands and Environments

4 bound ligands:
Ligand UDP 2 x UDP
Ligand EDO 3 x EDO
Ligand MN 2 x MN
Ligand A2G 2 x A2G
1 modified residue:
Ligand LPD 2 x LPD

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 50.241Å b: 80.445Å c: 71.949Å
α: 90° β: 93.19° γ: 90°
R-values:
R R work R free
0.178 0.175 0.235
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

4 citation in other articles

Structural and mechanistic insights into the cleavage of clustered O-glycan patches-containing glycoproteins by mucinases of the human gut.
Taleb et al. (2022)
3 more

2 mentions without citation

Molecular Recognition of GalNAc in Mucin-Type O-Glycosylation.
Sanz-Martinez et al. (2023)
1 more