› 7q54

Electron Microscopy
8.9Å resolution

Single Particle Cryo-EM structure of photosynthetic A4B4-glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia.

Released:
DOI: 10.2210/pdb7q54/pdb
PDB entry 7q54 coloured by chain, front view.
PDB entry 7q54 coloured by chain, side view.
PDB entry 7q54 coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Unravelling the regulation pathway of photosynthetic AB-GAPDH.
Marotta R, Del Giudice A, Gurrieri L, Fanti S, Swuec P, Galantini L, Falini G, Trost P, Fermani S, Sparla F
Acta Crystallogr D Struct Biol 78 1399-1411 (2022)
PMID: 36322422
Related structures: EMD-13825

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = 3-phospho-D-glyceroyl phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146344 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic Chains: A, C, O, Q
Molecule details ›
Chains: A, C, O, Q
Length: 451 amino acids
Theoretical weight: 48.18 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P12860 (Residues: 1-451; Coverage: 100%)
Gene names: GAPB, GPB1
Sequence domains:
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic Chains: B, D, P, R
Molecule details ›
Chains: B, D, P, R
Length: 402 amino acids
Theoretical weight: 43.16 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P19866 (Residues: 1-401; Coverage: 100%)
Gene names: GAPA, GPA1
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.9Å
Relevant EMDB volumes: EMD-13825

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