› 7q6x

X-ray diffraction
2.7Å resolution

OleP mutant S240Y in complex with 6DEB

Released:
DOI: 10.2210/pdb7q6x/pdb
PDB entry 7q6x coloured by chain, front view.
PDB entry 7q6x coloured by chain, side view.
PDB entry 7q6x coloured by chain, top view.
Source organism: Streptomyces antibioticus
Primary publication:
Point Mutations at a Key Site Alter the Cytochrome P450 OleP Structural Dynamics.
Montemiglio LC, Gugole E, Freda I, Exertier C, D'Auria L, Chen CG, Nardi AN, Cerutti G, Parisi G, D'Abramo M, Savino C, Vallone B
OpenAccess logo Biomolecules 12 (2021)
PMID: 35053203

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-561100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P-450 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 406 amino acids
Theoretical weight: 44.88 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59819 (Residues: 4-407; Coverage: 99%)
Gene name: oleP
Sequence domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
Ligand HEM 6 x HEM
Ligand DEB 6 x DEB
Ligand FMT 6 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: C2
Unit cell:
a: 248.624Å b: 110.355Å c: 160.046Å
α: 90° β: 129.63° γ: 90°
R-values:
R R work R free
0.207 0.204 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Tumor metabolic crosstalk and immunotherapy.
Zhang et al. (2023)
3 other articles

1 mention without citation

Point Mutations at a Key Site Alter the Cytochrome P450 OleP Structural Dynamics.
Montemiglio et al. (2021)