› 7q6y

X-ray diffraction
2.22Å resolution

The X-ray crystal structure of CbTan2, a tannase enzyme from Clostridium butyricum

Released:
DOI: 10.2210/pdb7q6y/pdb
PDB entry 7q6y coloured by chain, front view.
PDB entry 7q6y coloured by chain, side view.
PDB entry 7q6y coloured by chain, top view.
Source organism: Clostridium butyricum
Primary publication:
Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium butyricum.
Ristinmaa AS, Coleman T, Cesar L, Langborg Weinmann A, Mazurkewich S, Brändén G, Hasani M, Larsbrink J
OpenAccess logo J Biol Chem 298 101758 (2022)
PMID: 35202648

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BD-FAE-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 54.97 KDa
Source organism: Clostridium butyricum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6M0U600 (Residues: 1-490; Coverage: 100%)
Gene names: CBLFYP62_01769, FF104_18625
Sequence domains: BD-FAE

Ligands and Environments

2 bound ligands:
Ligand PEG 5 x PEG
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 90.675Å b: 95.003Å c: 131.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Potential Health Benefits of Gallic Acid: Therapeutic and Food Applications.
Hadidi et al. (2024)
2 other articles

1 mention without citation

Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium butyricum.
Ristinmaa et al. (2022)